Description
Recombinant full-length human METTL11A was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. The gene accession number is BC001396.
N-terminal Xaa-Pro-Lys N-methyltransferase 1 methylates (METTL11A) the N-terminus of target proteins with the N-terminal motif [Ala/Pro/Ser]-Pro-Lys when the initiator Met is cleaved. Specifically catalyzes mono-, di-or tri-methylation of exposed alpha-amino group of Ala or Ser residue in the [Ala/Ser]-Pro-Lys motif and mono-or di-methylation of Pro in the Pro-Pro-Lys motif. Some of the substrates may be primed by METTL11B-mediated monomethylation. Responsible for the N-terminal methylation of KLHL31, MYL2, MYL3, RB1, RCC1, RPL23A and SET. Required during mitosis for normal bipolar spindle formation and chromosome segregation via its action on RCC1